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. 2017 Apr 25;8:447. doi: 10.3389/fimmu.2017.00447

Figure 4.

Figure 4

Monocytes cultured in bronchial epithelial cell-conditioned media (BEC-CM) have an increased ability to trigger the differentiation of human naive T cells into IL-17 producers. Allogenic Naive T cells or Memory T cells were stimulated with conditioned monocytes [cultured with medium alone or with BEC-CM (BEC-cMo)] at a monocyte:T-cell ratio of 1:10 for 9 days, supplemented with IL-2 for the last 2 days and then stimulated for 5 h with PMA and ionomycin. Supernatants were analyzed for the release of (A) IL-17, (B) IFN-γ, and (C) IL-10. Data are expressed as means ± SEM of four to six independent experiments. Statistical analysis was performed using a Mann–Whitney test (*P < 0.05). (D) Production of IL-17 by naive T-cells primed with allogenic monocytes that were cultured with medium alone or with BEC-CM at a monocytes:T-cell ratio of 1:10. During the mixed lymphocyte reaction, cells were cultured in the presence of neutralizing antibodies to IL-1β, IL-6R, or both. Data are expressed as mean ± SEM of three independent experiments. Statistical analysis was performed using analysis of variance test (followed by Tukey multiple comparisons test) (*P < 0.05, **P < 0.01).