Figure 5.

Bronchial epithelial cell-conditioned media (BEC-CM) regulates the release of cytokines by monocytes and dendritic cells (DCs) differentially after lipopolysaccharide (LPS). Monocytes and DCs were cultured for 48 h with medium alone or with BEC-CM. Cells were stimulated with LPS (100 ng/mL) for the last 24 h. The secretion of cytokines was analyzed using the Luminex system or ELISA: (A) IL-12p70, (B) IL-10, (C) TNF-α, (D) IL-6, (E) IL-1β, and (F) IL-23. Data are expressed as means ± SEM of at least four independent experiments. Statistical analysis was performed using analysis of variance test (followed by the Fisher LSD test) (*P < 0.05, **P < 0.01).