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. 2017 Apr 25;8:707. doi: 10.3389/fmicb.2017.00707

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Copyright © 2017 Nazina, Shestakova, Semenova, Korshunova, Kostrukova, Tourova, Min, Feng and Poltaraus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rarefaction curves based on the results of comparison of the number of cloned 16S rRNA gene sequences and phylotypes in the DNA-derived clone libraries of Archaea (DA library, curve 1, 115 clones), in the DNA-derived library for Bacteria (DB, excluding planctomycetes, curve 2, 153 clones), in the RNA-derived library of Bacteria cDNA of 16S rRNA sequences (DR, curve 3, 151 clone), and in the total 16S rRNA gene library [DB + DP (89 clones)] + cDNA of 16S rRNA sequences of Bacteria (RB), curve 4, 393 clones. The sequences were grouped in phylotypes at the level of 97% between the sequences of the 16S rRNA genes or cDNA of 16S rRNA transcripts.