Table 2.
Diversity indices of the 16S rRNA gene sequences of Archaea and Bacteria and cDNA of 16S rRNA sequences of Bacteria in the clone libraries generated from DNA and RNA, respectively, of microorganisms of formation water from the high-temperature petroleum reservoir (December 2006).
| Parameter | Clone library | |||
|---|---|---|---|---|
| DNA-derived | RNA-derived | Bacteria total (DB + DP + RB) | ||
| Archaea | Bacteria | Bacteria | ||
| Library | DA | DB | RB | Btotal |
| Number of clones in the library | 115 | 153 | 151 | 393 |
| Number of phylotypes and their distribution within the library | 10 (44, 36, 16, 7, 7, 1, 1, 1, 1, 1) | 29 (100, 9, 4, 3, 3, 3, 3, 3, 2, 2, 2, 2, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1) | 27 (44, 36, 10, 10, 6, 6, 5, 5, 4, 3, 2, 2, 2, 2, 2, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1) | 52 (100, 57, 45, 37, 26, 11, 10, 10, 6, 6, 6, 5, 5, 4, 3, 3, 3, 3, 3, 3, 3, 3, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1) |
| Coverage (%) | 95.7 | 89.0 | 92.1 | 95.2 |
| Shannon-Weaver diversity index (H) | 1.55 | 1.71 | 2.40 | 2.80 |
| Evenness (H/Hmax) | 0.67 | 0.51 | 0.73 | 0.71 |
| Simpson's diversity index (1/S) | 3.68 | 2.30 | 6.31 | 8.60 |
| Chao1 (min, max 95% confidence interval) | 20 (12, 62) | 65 (39, 156) | 41 (31, 82) | 66 (57, 95) |
| Berger-Parker Dominance index (D) | 0.38 | 0.65 | 0.29 | 0.25 |