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Transactivation activity verification of FtbHLH3 in yeast. (A) The transformed cells were screened onto SD/-His-Trp medium. (B) After the galactosidase filter lift assay. F3: pBridge-bHLH3; Positive Control (PC): pBridge-GmMYBJ6; Negative Control 1(NC1): empty pBridge plasmid; Negative Control 2 (NC2): AH109 cells. Fusion proteins of pBridge-FtbHLH3, pBridge-GmMYBJ6 and pBridge were expressed in the yeast strain AH109.
