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. 2017 Apr 25;8:726. doi: 10.3389/fmicb.2017.00726

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Copyright © 2017 Krysenko, Okoniewski, Kulik, Matthews, Grimpo, Wohlleben and Bera.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HPLC/ESI-MS analysis of the glutamylated product generated by GlnA3 in in vitro assay. Two samples were analyzed in the MS positive mode: reaction mixtures without addition of GlnA3 (A) and with addition of GlnA3 (B). Extracted ion chromatograms for the GlnA3 reaction product corresponding to gamma-glutamylputrescine with charge to mass ratio of 218 m/z was shown (B), and no product in the sample without addition of GlnA3 could be detected (A).