FIGURE 4.

Transcriptional analysis of glnA3 in the presence of polyamines and ammonium as a sole nitrogen sources. Reverse transcriptase/PCR of glnA3 and hrdB (control) from S. coelicolor M145 cultivated in defined Evans medium with low (5 mM) or high (50 mM) concentrations of ammonium chloride or polyamines (Put – putrescine; Cad – cadaverine and Spd – spermidine, 25 mM of each) and glucose as a sole carbon source high Glc+ (25 g/l) or low Glc– (2.5 g/l). Total RNA was isolated from mycelium harvested after 24 h of cultivation in the defined Evans medium.