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Transcriptional analyses of putative gene homologs encoding predicted proteins involved in polyamine degradation in S. coelicolor. Reverse transcriptase/PCR of genes and hrdB (control) from S. coelicolor M145 cultivated in defined Evans medium with polyamines (Put – putrescine; Cad – cadaverine or Spd – spermidine, 25 mM of each) and glucose as a sole carbon source Glc (25 g/l). Total RNA was isolated from mycelium harvested after 24 h of cultivation in the defined Evans medium.
