Figure 4.

Immunofluorescence localization of ferroportin protein in the duodenum of suckling and weaned mice. Duodenal tissue was taken from 15-day-old and 25- to 28-day-old C57BL/6J mice and formalin-fixed sections were stained for ferroportin protein and imaged by confocal microscopy. Ferroportin protein is shown in red and nuclei is shown in blue (4’,6-diamidine-2’-phenylindole dihydrochloride). Intestinal-specific ferroportin knockout mice were used as a negative control to ensure antibody specificity. At least 6 sections were stained from each age group and representative images are shown. Fpn, ferroportin.