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. 2016 Mar;14(2):211–218. doi: 10.2174/1570161113666150722151817

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© 2016 Bentham Science Publishers

This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

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Fig. (2) — Inhibition of miR-135a on calcification, ALP activity and mRNA expression of Alkaline Phosphatase (ALP) and Osteocalcin (OC). A: Mineralized matrix formation was assessed by AlizarinRed S staining; B: Alkaline Phosphatase (ALP) activity was measured in cell cultures; C and D: The mRNA expression of Alkaline Phosphatase (ALP) or Osteocalcin (OC) was measured by real-time PCR. Cells were in passage 14. Values are expressed as mean ± SEM, n = 6 in each group.*P< 0.05, versus control group.