Figure 11.

Lactase protein is expressed in Gata4 cKI ileum. (A) Immunofluorescent staining showed Lactase protein (LCT, green) at the brush border in ileal epithelium of Gata4 cKI mice, whereas LCT protein was absent from ileal epithelium of control mice. Sections from at least 3 control and 3 Gata4 cKI animals were evaluated. E-cadherin (ECAD, red) was used to stain epithelial cell membranes. 4′,6-diamidino-2-phenylindole (DAPI) (blue) stained nuclei. Scale bars: 100 μm. (B) Immunoblot analysis of protein extracts from ileal epithelial cells of control and Gata4 cKI mice was used to quantify LCT protein (n = 3). To quantify protein expression, signal was measured using quantitative infrared immunoblotting (LI-COR) and National Institutes of Health ImageJ software. Arrowhead indicates the LCT band measured. LCT protein levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels. Molecular weight marker locations are indicated. Error bars show SEM. P values were determined by 2-sample Student t test: *P ≤ .05.