Table 2.
Strains and plasmids used in this study
| Strains | Relevant genotype | References |
|---|---|---|
| E. coli DH5α | ϕ80 dlacZΔM15Δ(lacZYA‐argF)U169 recA1 endA1 hsdR17 (rK− mK+) supE44 thi‐1gyrA relA1 | Laboratory stock |
| E. coli BL21 (DE3) | F− ompT gal dcm lon hsdSB(rB− mB−) λ(DE3 [lacI lacUV5‐T7 gene 1 ind1 sam7 nin5]) | Stratagene |
| E. coli JM109 | endA1, recA1, gyrA96, thi‐1, hsdR17 (rK− mK+), relA1, supE44, Δ(lac‐proAB), [F’ traD36,proAB, laqIqZΔM15] | New England Biolabs |
| E. coli KDZif1ΔZ | araD (gpt‐lac)5, rpsL (Strr), ΔspoS3::cat (Camr) [F’ lacIq (Z321(‐61) lacZYA*) Kanr] | (Vallet‐Gely et al., 2005) |
| E. coli BW25113 | F−, DE(araD‐araB)567, lacZ4787(del)::rrnB‐3, LAM−, rph‐1, DE(rhaD‐rhaB) 568, hsdR514 | Keio collection |
| E. coli JW4103 | F−, Δ(araD‐araB)567, ΔlacZ4787(::rrnB‐3), λ −, rph‐1, Δ(rhaD‐rhaB)568, ΔgroL768::kan, hsdR514 | Keio collection |
| L. crescens BT‐1 | Standard strain (Wild Type). | (Leonard et al., 2012) |
| S. meliloti 1021 | expR102::ISRm2011‐1expR.Smr | (Galibert et al., 2001) |
| FL01 | KDZif1ΔZ pACTR‐Zif, pBRGP‐ω. Tetr, Ampr. Kanr | This work |
| FL02 | KDZif1ΔZ pACTR‐CLIBASIA_03135‐Zif, pBRGP‐ω. Tetr, Ampr. Kanr | This work |
| FL03 | KDZif1ΔZ pACTR‐Zif, pBRGP‐CLIBASIA_03135‐ω. Tetr, Ampr. Kanr | This work |
| FL04 | KDZif1ΔZ pACTR‐CLIBASIA_03720‐Zif, pBRGP‐CLIBASIA_03135‐ω. Tetr, Ampr. Kanr | This work |
| FL05 | KDZif1ΔZ pACTR‐CLIBASIA_03135‐Zif, pBRGP‐CLIBASIA_03720‐ω. Tetr, Ampr. Kanr | This work |
| FL06 | KDZif1ΔZ pACTR‐CLIBASIA_03720‐Zif, pBRGP‐ω. Tetr, Ampr. Kanr | This work |
| FL07 | KDZif1ΔZ pACTR‐Zif, pBRGP‐CLIBASIA_03720‐ω. Tetr, Ampr. Kanr | This work |
| Plasmids | Characteristics | Reference |
| p15TV‐L | Expression vector Ampr. | (Pagliai et al., 2010) |
| pBRGP‐ω | Translational fusion vector | (Vallet‐Gely et al., 2005) |
| pACTR‐AP‐Zif | Translational fusion vector. Tetr | (Vallet‐Gely et al., 2005) |
| pBAD24 | Expression vector, arabinose inducible promoter, Ampr | (Guzman et al., 1995) |
| pFL02 | pACTR‐CLIBASIA_03135‐Zif. Tetr | This work |
| pFL03 | pBRGP‐CLIBASIA_03135‐ω. Ampr | This work |
| pFL04 | pBRGP‐CLIBASIA_03720‐ω. Ampr | This work |
| pFL05 | pACTR‐CLIBASIA_03720‐Zif. Tetr | This work |
| pFL06 | pBAD24‐CLIBASIA_03135. Ampr | This work |