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. 2017 Apr 25;7:46716. doi: 10.1038/srep46716

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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PMA-differentiated THP-1 cells (2 × 10⁶/well/2 ml) were infected with recombinant Ms_Vec and Ms_PE_PGRS41 strains. After 6, 24, and 48 h infection, the infected cells and supernatant were collected. RT-PCR was used for analyze TNF-α mRNA (A), IL-6 mRNA (B), IL-1β mRNA (C). ELISA was used for detect the production of TNF-α (D), IL-6 (E), IL-1β (F).