Figure 4. Production of secreted mediators by TPH-1 human macrophages incubated for 24 h with 1, 10 or 100 μg/ml of secreted polysaccharides (secreted-PS) from C. neoformans or C. liquefaciens.

Secreted mediator levels in culture supernatants were determined using the Magpix xMAP multiplex system (Biorad Laboratories Inc., Hercules, CA, USA). Control, untreated cells; + Control, cells incubated with 20 ng/mL TNFα. Data represent mean ± SEM of 4 independent experiments. **p < 0.01 relative to cells challenged by 100 μg/ml C. neoformans vs. C. liquefaciens (Student’s t-test).