Fig. 1.

Metformin suppressed the insufficient RFA-induced proliferation, migration and invasion of HepG2 cells. HepG2 cells were treated with insufficient RFA (47 °C 5, 10, 15, 20 and 25 min) gradually. Residual HepG2 (named as HepG2-H) cells were collected and used for the next experiments. a The effect of metformin on proliferation rate of HepG2 and HepG2-H cells was evaluated by MTT assay. Error bars represent the SEM of data obtained in five independent experiments. b Colony formation ability of HepG2 and HepG2-H cells after the treatment of metformin was assessed. c The effect of metfromin on migration and invasion of HepG2 and HepG2-H cells were shown. Error bars represent the SEM of data obtained in three independent experiments. ns no significance