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. 2017 Apr 24;16:69. doi: 10.1186/s12934-017-0687-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Expression of Zhd101 in Lactobacillus reuteri cells. a RT-PCR for Zhd101 in Lb. reuteri Pg4, Lb. reuteri pNZ-zhd101, and Lb. reuteri pNZ3004, along with the housekeeping gene 16S rRNA. b Western blot for purified recombinant Zhd101 and Zhd101 in the intracellular extracts of Lb. reuteri Pg4, Lb. reuteri pNZ3004, and Lb. reuteri pNZ-zhd101. The samples (2 μg of protein in each lane) were separated by SDS-PAGE with a 12.5% gel and probed with mouse polyclonal anti-zhd101 antibody and horseradish-peroxidase-linked anti-mouse IgG antibody as the primary and secondary antibodies, respectively