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. 2017 Apr 24;16:70. doi: 10.1186/s12934-017-0688-7

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effects of deficiency or over-expression of sppA and tepA on the extracellular secretion. a The concentrations of total extracellular proteins of different strains; b SDS-PAGE analysis of the extracellular proteins from different host strains. M protein marker (95, 72, 55, 43, 34 kDa); Lane 1 B. licheniformis BL10; Lane 2 B. licheniformis BL10T; Lane 3 B. licheniformis BL10S; Lane 4 B. licheniformis BL10GT; Lane 5 B. licheniformis BL10GS. Data are represented as the means of three replicates and bars represent the standard deviations, *P < 0.05; and **P < 0.01 indicate the significance levels between recombinant strains and control strain