Table 1.
The strains and plasmids used in this study
| Strains | Relevant properties | Source of reference |
|---|---|---|
| Escherichia coli | ||
| DH5α | supE44 ΔlacU169 (f 80 lacZΔM15) hsd R17 recA1 gyrA96 thi1 relA1 | [7] |
| Bacillus licheniformis | ||
| WX-02 | Polyglutamate productive strain (CCTCC M208065) | CCTCC |
| BL10 | WX-02(Δmpr, Δvpr, ΔaprX, Δepr, Δbpr, ΔwprA, ΔaprE, ΔbprA, Δhag, ΔamyL) | [7] |
| BL10/pHY-amyL | BL10 harboring pHY-amyL | This study |
| BL10/pP43SacCNK | BL10 harboring pP43SacCNK(CCTCC M2014253) | CCTCC |
| BL10T | BL10(ΔtepA) | This study |
| BL10S | BL10(ΔsppA) | This study |
| BL10T/pHY-amyL | BL10T harboring pHY-amyL | This study |
| BL10S/pHY-amyL | BL10S harboring pHY-amyL | This study |
| BL10T/pP43SacCNK | BL10T harboring pP43SacCNK | This study |
| BL10S/pP43SacCNK | BL10S harboring pP43SacCNK | This study |
| BL10GT | Overexpression of tepA in BL10 | This study |
| BL10GS | Overexpression of sppA in BL10 | This study |
| BL10GT/pHY-amyL | BL10GT harboring pHY-amyL | This study |
| BL10GS/pHY-amyL | BL10GS harboring pHY-amyL | This study |
| BL10GT/pP43SacCNK | BL10GT pP43SacCNK | This study |
| BL10GS/pP43SacCNK | BL10GS pP43SacCNK | This study |
| Plasmids | This study | |
| T2(2)-ori | Bacillus knockout vector; Kanr | [7] |
| T2-sppA | T2(ori)-sppA(A + B); to knock out sppA | This study |
| T2-tepA | T2(ori)-tepA(A + B); to knock out tepA | This study |
| T2-GsppA | T2(ori)-sppA(A + B+sppA); to over-express sppA | This study |
| T2-GtepA | T2(ori)-tepA(A + B+tepA); to over-express tepA | This study |
| pHY300PLK | E. coli and B. s shuttle vector; Ampr, Tetr | [7] |
| pP43SacCNK | PHY300PLK + Promotor-P43 (B. subtilis 168) + signal peptide of SacC (B. subtilis 168) + aprN(B. subtilis MBS 04-6) + Terminator of amyL (B. licheniformis WX-02) | [7] |
| pHY-amyL | PHY300PLK + Promotor-P43 (B. subtilis 168) + amyL (containing its own signal peptide and terminator, B. licheniformis WX-02) | This study |