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. 2017 Apr 24;18:19. doi: 10.1186/s12865-017-0202-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — EMT-associated cellular marker alterations. a E-Cadherin (epithelial cell marker) protein levels at 24 h after scratching (n = 4). b Vimentin (mesenchymal cell marker) protein levels at 24 h after scratching (n = 4). c The nucleus/cytosol ratio of Snail 1 (EMT-related transcriptional factor) protein levels at 24 h after scratching (n = 4). d Immunoreactivities of E-cadherin (green), vimentin (green), and Snail 1 (green) at 24 h after the scratch (Blue; DAPI) 24 h after poly I:C stimulation with or without anti-IL-8 antibody. Data are presented as the mean ± SEM. n.s.: not significant according to the Tukey-Kramer test. Data for immunofluorescence assay are representative of at least three independent experiments. Scale bar: 100 μm