Skip to main content
. Author manuscript; available in PMC: 2017 Apr 25.
Published in final edited form as: Cell Rep. 2017 Mar 14;18(11):2584–2591. doi: 10.1016/j.celrep.2017.02.062

Figure 4. Conditional knockout of VGLUT2 from DA neurons does not affect the acquisition of ICSS or place preference.

Figure 4

(A) and (B) Active lever pressing was reinforced with VTA optogenetic stimulation (50 Hz, 8 pulses with 1 ms pulse width; 8 mW) in sessions 1–5 and then with optogenetic stimulation of increased intensity (3 ms pulse width; 32 mW) in sessions 6–10. In session 10, 11 out of 14 cKO and 12 out of 16 control mice responded 500 times or more.

(C) Mean responses with SEM are shown with the data of responders with 500 responses or more. The cutoff of 500 responses is to focus on differences in responders of the two groups and is justified by the fact that similar ratios of mice were excluded from the analyses between the two groups. * P < 0.05, a significant group x session interaction.

(D) and (E) While no optogenetic stimulation was delivered in sessions 1 or 5, mice received VStr optogenetic stimulation in one of two compartments during sessions 2–4. Depicted are percent time spent in the stimulation compartment of each mouse.

(F) Percentage of time (Mean ± SEM) spent in the stimulation compartment.