Figure 3.

Mitochondrial import of the wild-type StAR, N-62 StAR and N-30 StAR. ³⁵S-methionine-labeled (A) wild-type StAR, (B) N-62 StAR and (C) N-30 StAR were synthesized in a cell-free rabbit reticulocyte lysate system (CFS), and then, the newly synthesized protein was imported into mitochondria isolated from MA-10 cells. The imported and unimported fractions were separated by washing (Wash) followed by centrifugation, where the unimported fraction remained in the supernatant (S), and the imported fraction remained in the pellet (P). Sodium carbonate (Carb) determines the lipid integrated from the unintegrated protein.