TABLE 1.
Primers used in this study
| Primer | Target organism | Orientation (5′→3′) | Sequence (5′→3′) | Purpose | Reference |
|---|---|---|---|---|---|
| FKS1-453-URA3F | S. cerevisiae | Sense | AAAGAGACCCGTACTTGGTTACATTTGGTCACCAACTTCAGAGTGCACCATACCACAGCT | Generation of S. cerevisiae FKS1-deleted mutant | 8 |
| FKS1-649-URA3R | S. cerevisiae | Antisense | GTATTCACCTGTACACCTCATTGCAGTGGTGGACAAAATTGGTATTTCACACCGCATAGG | Generation of S. cerevisiae FKS1-deleted mutant | 8 |
| URA3iR | S. cerevisiae | Antisense | TGCCTTTAGCGGCTTAACTG | Confirmation of S. cerevisiae FKS1 deletion | 8 |
| FKS1-375 | S. cerevisiae | Sense | GGTCGTTTTGTCAAGCGTGA | Confirmation of S. cerevisiae FKS1 deletion, chimeric FKS1 construction, and S. cerevisiae FKS1 reconstruction | 8 |
| FKS1-707R | S. cerevisiae | Antisense | ATTTCCCAACAGAGAAAATGG | S. cerevisiae FKS1 reconstruction | 8 |
| LMDM85 | S. cerevisiae | Antisense | CGTAGTGAGGAGTCAATACTGTG | Chimeric FKS1 construction | This study |
| Fus5-FKS1-Sc-CguiF | S. cerevisiae and C. guilliermondii | Sense | CGTAGATTCTGGTTTTTATGCATCATCTTCGTGGTTAACTTGGCCCC | Chimeric FKS1 construction | This study |
| Fus5-FKS1-Sc-CguiR | S. cerevisiae and C. guilliermondii | Antisense | GGGGCCAAGTTAACCACGAAGATGATGCATAAAAACCAGAATCTACG | Chimeric FKS1 construction | This study |
| Fus3-FKS1-Sc-CguiF | S. cerevisiae and C. guilliermondii | Sense | GTACAGAGAACATTTGTTGGCTATTGACCATGTACAAAAATTACTATATC | Chimeric FKS1 construction | This study |
| Fus3-FKS1-Sc-CguiR | S. cerevisiae and C. guilliermondii | Antisense | GATATAGTAATTTTTGTACATGGTCAATAGCCAACAAATGTTCTCTGTAC | Chimeric FKS1 construction | This study |
| FKS1-305F | S. cerevisiae | Sense | CCCTGGAAAGAGTTCGTCATATC | Confirmation of FKS1 chimeric reconstitution | This study |
| Cg/h-R | C. guilliermondii | Antisense | CAAACCACCCAAAGGCATAAC | Confirmation of FKS1 chimeric reconstitution | This study |