TABLE 2.
Antibiotic resistance genes carried by E. coli isolates
| Isolate group and genea | Location of gene (Inc group, size [kb])b | Resistancec |
|---|---|---|
| ST-32 isolates | ||
| blaCTX-M-15 | Plasmid (F1B, 113.1) | β-Lactams (extended spectrum) |
| blaTEM-1B | Plasmid (F1B, 113.1) | β-Lactams (penicillin) |
| aph(6)-Ia (strA) | Plasmid (F1B, 113.1) | Aminoglycosides (streptomycin) |
| aph(6)-Id (strB) | Plasmid (F1B, 113.1) | Aminoglycosides (streptomycin) |
| qnrS1 | Plasmid (F1B, 113.1) | Quinolones |
| sul2 | Plasmid (F1B, 113.1) | Sulfonamides |
| tet(A) | Plasmid (F1B, 113.1) | Tetracyclines |
| dfrA14 | Plasmid (F1B, 113.1) | Trimethoprim |
| ST-617 isolates | ||
| ant(3″)-Ia (aadA1) | Plasmid (R, 67.9) | Aminoglycosides (streptomycin) |
| ant(3″)-Ia (aadA2) | Plasmid (R, 67.9) | Aminoglycosides (streptomycin) |
| blaCTX-M-15 | Chromosome | β-Lactams (extended spectrum) |
| blaTEM-1B | Plasmid (I1, 102.3–208) | β-Lactams (penicillin) |
| blaPSE-1 (blaCARB-2) | Plasmid (R, 67.9) | β-Lactams (penicillin and carbenicillin) |
| erm(42) | Plasmid (I1, 102.3–208) | Macrolides |
| mcr-1 | Plasmid (I2, 64.5) | Polymyxins |
| cmlA1 | Plasmid (R, 67.9) | Phenicols |
| floR | Plasmid (I1, 102.3–208) | Phenicols |
| sul3 | Plasmid (R, 67.9) | Sulfonamides |
| tet(A) | Plasmid (R, 67.9) | Tetracyclines |
| dfrA16 | Plasmid (R, 67.9) | Trimethoprim |
a
Based on the closest match obtained using the BLAST algorithm (http://blast.ncbi.nlm.nih.gov). The mcr-1 data discussed in the present study are shown in bold.
b
Based on long-read sequence analysis. The plasmid incompatibility group and approximate size are provided where relevant.
c
Predicted resistance phenotype. Note that for clarity, where detailed, only clinically relevant antibiotics are listed.