FIG 1.

Genetic organization of Tn6350 (GenBank accession number KY347015), which harbors genes encoding pyocin S8. Predicted coding regions are represented by thick arrows indicating the direction of transcription. This element contains eight ORFs, including an ATPase (orf1), a transposase (int), a resolvase (tnpR), a cytotoxic subunit of pyocin S8 (pys8), an immunity subunit of pyocin S8 (imm), and three more hypothetical proteins (ofr4, orf7, and orf8). The inverted repeat (IR) sequences flanking the element are marked by black triangles (IRL, inverted repeat left; IRR, inverted repeat right). They have 48 bp and 100% identity. The element is flanked by short direct repeats (DRs) rich in CG dinucleotide.