FIG 4.

Susceptibility of mutants to caspofungin (CAS) compared to that of parental strains and strains with duplicated Ch5. The results shown are for spot assays of growth on control YPD medium as well as YPD medium supplemented with caspofungin, as indicated. The time of incubation at 37°C is indicated. Strains are indicated on the left. Names of the derivatives are shown under the names of the corresponding parental strain (JRCT1 or SC5314). The monosomic (1n), duplicated (2n), or iso-Ch5R condition of Ch5 is indicated. Note that Ch5 monosomic mutants demonstrated less growth than related disomic strains on YPD control medium. However, this pattern was reversed in the presence of caspofungin.