TABLE 2.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli | ||
| OmniMAX | Strain used in transformation; F′ {proAB+ lacIq lacZΔM15 Tn10(Tetr)} mcrA Δ(mrr-hsdRMS-mcrBC) Φ80(lacZ)ΔM15 Δ(lacZYA-argF) U169 endA1 supE44 thi-1 gyrA96 relA1 deoR tonA panD | Invitrogen, Life Technologies |
| TGI | Strain used in transformation; supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK− mK−) [F′ traD36 proAB+ lacIq ZΔM15] | 61 |
| CC118λpir | Donor cell in conjugation; strain CC118 lysogenized with λ pir phage (Tc′) Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE (Am) recA1 | 56 |
| 1047 (pRK2013) | Helper cell in conjugation harboring pRK2013 (Kanr) plasmid | 62 |
| S. maltophilia | ||
| D457 | Clinical strain | 63 |
| MBS287 | D457-derived mutant (SmeRv G266N) overexpressing SmeVWX efflux pump | 22 |
| MBS704 | D457 ΔsmeW | This work |
| PBT02 | D457 with pPBT04 plasmid | This work |
| PBT06 | MBS287 with pPBT04 plasmid | This work |
| Plasmids | ||
| pGEM-T Easy vector | Cloning vector, Ampr | Promega |
| pSEVA237Y | Plasmid containing YFP; replication origin pBBR1; Kanr | 24 |
| pPBT02 | pGEMT-derived plasmid containing smeVWX promoter region | This work |
| pPBT04 | pSEVA237Y-derived plasmid containing smeVWX promoter region | This work |
| pEX18Tc | Gene replacement vector; sacB, Tcr | 23 |
| pBS51 | pGEMT-derived plasmid containing the 5′ and 3′ regions of smeW gene | This work |
| pBS52 | pEX18Tc-derived plasmid containing the 5′ and 3′ regions of smeW gene | This work |
a
Tetr, tetracycline resistance; Kanr, kanamycin resistance; Ampr, ampicillin resistance.