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Atovaquone resistance affects the fitness of growth of mutant strain Y268C. Parasite growth rates were determined by qRT-PCR as described in Materials and Methods. (a) Growth measurements for the wild-type strain (9.46 ± 1.10 per 24 h) (top) and an atovaquone mutant strain with a Y268C amino acid substitution (2.92 ± 0.61 per 24 h) (bottom). (b) Data from qRT-PCR were validated by microscopic measurement of parasitemia levels for the wild-type strain over a period of 6 days.
