TABLE 2.

Primers and conditions used in RT-qPCR^a

Primer	Sequence (5′-3′)	Cycling conditions
phoP_F	ATTGAAGAGGTTGCCGCCCGC	95°C for 1 s, 52°C for 5 s, 72°C for 7 s
phoP_R	GCTTGATCGGCTGGTCATTCACC	95°C for 1 s, 52°C for 5 s, 72°C for 7 s
phoQ_F	ATATGCTGGCGAGATGGGAAAACGG	95°C for 1 s, 52°C for 5 s, 72°C for 7 s
phoQ_R	CCAGCCAGGGAACATCACGCT	95°C for 1 s, 52°C for 5 s, 72°C for 7 s
pmrK_FT	GCGGGCCATCAGGATCGACAGCG	95°C for 1 s, 65°C for 5 s, 72°C for 7 s
pmrK_RT	CGTTCTGGTACTACATCCCGTTCCTGA	95°C for 1 s, 65°C for 5 s, 72°C for 7 s
rpsL13_F	GCCGTACTTGGAGCGAGCCTG	95°C for 1 s, 52°C for 5 s, 72°C for 7 s
rpsL14_F	CCGTGGCGGTCGTGTTAAAGA	95°C for 1 s, 52°C for 5 s, 72°C for 7 s

^aReprinted from reference 21 with permission.