TABLE 1.
Kinetic parameters for PAS uptake by stably transfected HEK-OATP1B1, HEK-OCT1, HEK-OCT2, HEK-OAT1, and HEK-OAT3 cellsa
| Transporter | Km (μM) | Vmax (pmol/min/mg protein) |
|---|---|---|
| OATP1B1 | 50.0 ± 13.6 | 129.7 ± 17.5 |
| OCT1 | 20.3 ± 4.6 | 48.7 ± 12.2 |
| OCT2 | 28.7 ± 6.8 | 70.7 ± 9.8 |
| OAT1 | 78 ± 18.2 | 1,898 ± 112 |
| OAT3 | 100.0 ± 23.6 | 1,572 ± 207 |
a
The intracellular kinetics of PAS uptake were derived from an in vitro experiment with PAS at concentrations ranging from 1 to 400 μM, and the data are normalized to those for the control. Data are presented as the means ± SDs from three or more independent experiments. Km, Michaelis-Menten constant; Vmax, maximum rate of uptake.