Table 6. Selenobiotinyl streptavidin crystallography figures of merit.

	Selenobiotinyl Streptavidin
Data collection	PDB ID (5JD2)
Beamline	LCLS (CXI)
Space group	P21
Cell dimensions
a, b, c (Å)	50.7, 98.4, 53.1
α, β, γ (°)	90, 112.7, 90
Resolution (Å)	32.51–1.90 (1.97–1.90)
Rsplit	0.048 (0.395)
I/σ(I)	14.0 (2.7)
Completeness (%)	1.0 (1.0)
SFX multiplicity of observations	1447.6 (1003.3)
CC*	1.000 (0.930)
CC1/2	0.998 (0.762)
CCano	0.177 (0.003)
Wilson B Factor (Å2)	29.58
Refinement
No. reflections	38,327 (3,817)
Rwork/Rfree	0.166/0.199 (0.231/0.253)
Ramachandran favored (%)	89.3
Ramachandran allowed (%)	10.5
Ramachandran outliers (%)	0.2
No. atoms
Protein	3,630
Ligand/Ion	64
Water	265
B-factors (Å2)
Protein	34.0
Ligand/Ion	38.9
Water	43.5
R.m.s. deviations
Bond lengths (Å)	0.006
Bond angles (°)	1.04