Figure 2. Example of the use of the proposed subset of strains representing the cyanobacterial ‘tree of life’ (see Subset_Condens_Tree in the Data Records section and Phylogenetic analyses in Methods) to evaluate the phylogenetic placement of strains not included in Supplementary Fig. 1 due to having short 16S rRNA gene sequences (in bold).

The evolutionary history was inferred by using the Maximum Likelihood method based on the GTR+G+I model. Bootstrap values indicated near internal branches; values below 50% were omitted. Information for each cyanobacterial strain include accession number of the nucleotide sequence, strain ID, eventual taxonomic synonyms or other strain names (in parentheses), and co-identical strains or other strain codes (in parentheses). Letters after colon indicate the categorization of strains as follows (see also Strain_Category in Data Record section): T, Type strain of the Type species; t, not the type strain, but phylogenetically close-related; R, Reference strain in Bergey's Manual of Systematic Bacteriology⁹; r, not the reference strain, but phylogenetically close-related; G, strain with its genome sequenced and publicly available; E, strain studied from exsiccata. A letter in parentheses means that there is a taxonomic-related uncertainty with the taxon name (see taxonomic comments) or the assigned strain’s category couldn’t be yet fully confirmed (e.g., for provisional species names). The outgroup was pruned from the tree for clarity. The scale bar represents nucleotide substitutions per site.