Figure 3.

Serum anti-OVA IgG levels in mice immunized with OVA/Alhydrogel® vaccine dry powder that was stored at 40° C, 30° C, or room temperature for 1 month (A) or 3 months (B) and then reconstituted. As controls, mice were injected with sterile PBS or freshly prepared OVA/Alhydrogel® vaccine (i.e., Fresh vaccine). Female BALB/c mice (n = 5) were injected (s.c.) on days 0, 14, and 28 with 5 μg of OVA per mouse. Total anti-OVA IgG levels in serum samples were measured about 3 weeks after the third dose. Data are mean ± SD (n.s., not significant).