Figure 5.

Representative particle size distribution curves of OVA/Alhydrogel® vaccine before (i.e., OVA/Alhydrogel®, FV, □) and after it was subjected to thin-film freezing on a pre-cooled rotating metal drum (OVA/Alhydrogel®, TFFD, ∘), thin-film freeze-drying on the inner surface of a silalized glass vial (OVA/Alhydrogel®, single vial TFFD, Δ), or conventional tray-freezing (OVA/Alhydrogel®, Tray freezing, ◊), and then lyophilization and reconstitution (FV, fresh vaccine; TFFD, thin-film freeze-drying). The experiment was performed with 3 replicates with similar results.