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. 2016 Dec 14;13(4):816–822. doi: 10.1080/21645515.2016.1261229

Figure 4.

Figure 4.

BCG priming and VSV-846 boosting increased the percentage of polyfunctional (T)cells. Mouse splenocytes 24 weeks after the BCG challenge were isolated and the cells were stained with specific cytokine antibodies and subjected to flow cytometry. (A) Percentage of polyfunctional T cells producing IFN-γ, TNF-a, or IL-2 in total CD4+ T cells. (B) Pie chart analysis of proportions of all single-, double-, and triple-positive CD4+ T cells. Results were expressed as the mean ± SD (n = 6) from three independent experiments. ***P < 0.001.