Figure 2. Electrophysiological characteristics of adherent and suspended Dopa. 4U neurons.
Manual measurements of ion channel properties applying progressive 500-ms wide 5-mV square-shaped steps from −100 mV to 60 mV. I-V relationship of NaV and delayed KV currents for adherent (a) compared to suspended (b) Dopa.4U neurons. Please note the −15-mV abscissa shift, resulting from the liquid junction potential. Measurements indicated that suspended and adherent cells had the same ion channel compositions, but with altered current magnitudes. (c) Effects of Accutase, Detachin or Trypsin-EDTA 1X treatment on the gigaseal rate (white columns) and on the success rate (gigasealed cells with both, delayed KV and NaV currents; striped columns). The number of experiments is displayed above the bars. (d,e) Magnitudes of the delayed KV and NaV currents for the adherent (white columns) and suspended (gray columns) cells before and after harvesting at DIV 8 (nadherent=3, nsuspended=9), 12 (nadherent=3, nsuspended=10), or 13 (nadherent=4, nsuspended=9). All error bars are defined as s.e.m.