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qRT-PCR validation of 16 randomly selected differentially expressed miRNAs identified by small RNA sequencing. a miRNAs expressed in YA05-17. b miRNAs expressed in ROC22. Sugarcane buds of ROC22 and YA05-179 post inoculation with sterile water or Sporisorium scitamineum for 48 h were used as qRT-PCR samples. The data of qRT-PCR were normalized to the 5S rRNA expression level and represented as means of three replicates (n = 3) ± standard error
