Fig. 5.

Oral microbiota influences the expansion of γδT17 cells in the draining cLNs. a The oral microbiota from WT and IL-17r^−/− mice was analyzed by sequencing 16S rRNA gene (v1–v3 region) and analyzed using QIIME 1.9.0 software platform. The abundant phylum and genus distribution between WT and IL-17r^−/− suggest alterations in the microbial composition. Principle coordinate analysis of unweighted UniFrac distance matrix showing the distinct clusters for WT (blue) and IL-17r^−/− (red) mice. ** p < 0.001; *** p < 0.0001. b cLNs (upper) and iLNs (lower) after oral antibiotics treatment for 6 weeks along with the corresponding weight of each node and the total cell number from that LNs. Representative of 2-3 experiments. ***p < 0.001. c Flow cytometry analysis showing percentages of γδ T cells, αβT cells, Vγ6 γδ T cells, and IL-17-producing γδ T and αβ T cells in the cLNs after 6 week oral antibiotics treatment from 1–2 days prior to birth to 6 weeks of age. Representative of two to three experiments. *p < 0.05, **p < 0.01, ***p < 0.001. d Flow cytometry analysis showing percentages of γδ T cells, Vγ6 γδ T cells, γδT17, and Th17 in the lungs, spleen, and colon (lamina propria) after 6-week oral antibiotics treatment from 1–2 days prior to birth to 6 weeks of age. Representative of two to three experiments. *p < 0.05, **p < 0.01, ***p < 0.001