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. 2017 Apr 25;12(4):e0176221. doi: 10.1371/journal.pone.0176221

Fig 4. Directional transcription is required for plasmid interference.

Fig 4

The BL21AI strain containing the LLA Csm module plasmid was challenged with five different target plasmids. (A) The target plasmids contain the LLA target sequence (yellow), a heterologous sequence (grey) or no target sequence (not shown in panel A). The LLA target (yellow) was oriented relative to the Trc promoter (blue) to effect transcription of either an RNA complementary to the crRNA (LLA-f) or the reverse complement (LLA-r). Transcription of the LLA target sequence was inhibited by flanking transcription terminators (red T) in the t-LLA-f-t target plasmid. (B) Plasmid targeting assay was performed as in Fig 2. Representative results of 3 experiments are shown.