REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
FITC-conjugated anti-BrdU antibody	BioLegend	Cat# 364104 RRID:AB_2564481
rabbit polyclonal anti-cyclin D1	Santa Cruz Biotechnology	Cat# sc-753 RRID:AB_2070433
mouse monoclonal anti-Cyclin D3	Cell Signaling Technology	Cat# 2936 RRID:AB_2070801
polyclonal anti-p53	Santa Cruz Biotechnology	Cat# sc-6243 RRID:AB_653753
rabbit monoclonal anti-p21	Cell Signaling Technology	Cat# 2947 RRID:AB_823586
rabbit monoclonal anti-p27	Cell Signaling Technology	Cat# 3686 RRID:AB_2077850
mouse monoclonal anti-Cyclin A2	Cell Signaling Technology	Cat# 4656 RRID:AB_2071958
mouse monoclonal anti-Cyclin E1	Cell Signaling Technology	Cat# 4129 RRID:AB_2071200
rabbit monoclonal anti-p-Akt(S473)	Cell Signaling Technology	Cat# 4060 RRID:AB_2315049
rabbit monoclonal anti-p-GSK-3β(S9)	Cell Signaling Technology	Cat# 5558 RRID:AB_10013750
mouse monoclonal anti-Aurora A	Santa Cruz Biotechnology	Cat# sc-373856 RRID:AB_10988868
rabbit polyclonal anti-cyclin A1	Santa Cruz Biotechnology	Cat# sc-7252 RRID:AB_1562274
rabbit monoclonal anti-GAPDH	Cell Signaling Technology	Cat# 2118 RRID:AB_561053
rabbit monoclonal anti-α-Tubulin	Cell Signaling Technology	Cat# 2125 RRID:AB_2619646
goat peroxidase-conjugated anti-rabbit	Cell Signaling Technology	Cat# 7074 RRID:AB_2099233
horse peroxidase-conjugated anti-mouse	Cell Signaling Technology	Cat# 7076 RRID:AB_330924
Bacterial and Virus Strains
One Shot® BL21(DE3) Chemically Competent/E. coli/	Invitrogen (Thermo Fisher Scientific)	Cat#C600003
Chemicals, Peptides, and Recombinant Proteins
THIAZOLYL BLUE TETRAZOLIUM BROMIDE, MTT	Sigma Aldrich	Cat#M5655-1G; CAS: 298-93-1
Dimethyl sulfoxide, DMSO, Hybri-Max (for cell treatment and cryopreservation)	Sigma Aldrich	Cat#D2650-5X10ML; CAS: 67-68-5
5-Bromo-2′-deoxyuridine (BrdU) BioUltra, ≥99%	Sigma Aldrich	Cat#B9285-50MG; CAS: 59-14-3
Staurosporine	Santa Cruz Biotechnology	Cat# sc-3510 A; CAS: 62996-74-1
Propidium Iodide (PI)	Serva	Cat#33671.01; CAS: 25535-16-4
Hoechst 33342	Thermo Fisher Scientific	Cat#62249; CAS: 23491-52-3
RIPA buffer	Sigma Aldrich	Cat#R0278-50ML
Protease inhibitor cocktail	Sigma Aldrich	Cat#P8340-5ML
Phosphatase inhibitor cocktail	Roche	Cat#04 906 845 001
Albumin, Bovine, Fraction V. Heat Shock Isolation (BSA)	BioShop	Cat#ALB001.250
Clarity Western ECL Substrate	BioRad	Cat#1705061
Lipofectamine 2000	Life Technologies	Cat#11668-027
Renozol	GenoPlast Biochemicals	Cat#BMGPB1100-2
M-MLV Reverse Transcriptase	Promega	Cat# M1701
Hydroxylamine solution 50 wt. % in H2O	Sigma Aldrich	Cat# 438227; CAS: 7803-49-8
Lithocholic acid	Sigma Aldrich	Cat# L6250; CAS: 434-13-9
Glycine	Sigma Aldrich	Cat# 410225; CAS: 56-40-6
Ammonium hydroxide solution	Sigma Aldrich	Cat# 09859; CAS: 13550-49-7
4-(2-Aminoethyl)morpholine	Sigma Aldrich	Cat# A55004; CAS: 2038-03-1
Acetyl chloride	Sigma Aldrich	Cat# 320129; CAS: 75-36-5
Chromium(VI) oxide	Sigma Aldrich	Cat# 675644; CAS: 1333-82-0
Morpholine	Sigma Aldrich	Cat# 394467; CAS: 110-91-8
Isobutyl chloroformate	Sigma Aldrich	Cat# 177989; CAS: 543-27-1
DIC	Sigma Aldrich	Cat# D125407; CAS: 693-13-0
Chloroform-d	Armar	Cat# 013300.2040; CAS: 865-49-6
DMSO-d6	Armar	Cat# 015600.2035; CAS: 2206-27-1
Sulfuric acid	POCH	Cat# 575000115; CAS: 7664-93-9
Acetic acid	POCH	Cat# 568760421; CAS: 64-19-7
Methanol	POCH	Cat# 621990426; CAS: 67-56-1
Dichloromethane	POCH	Cat# 628410421; CAS: 75-09-2
TEA	POCH	Cat# 848930423; CAS: 121-44-8
Acetonitrile	POCH	Cat# 102640111; CAS: 75-05-8
Sodium Bicarbonate	POCH	Cat# 810530115; CAS: 144-55-8
Sodium Sulfate Anhydrous	POCH	Cat# 807870111; CAS: 7727-73-3
LB BROTH (MILLER)	BioShop	Cat#LBL407.5
IPTG, Reagent Grade, min 99%	BioShop	Cat#IPT002.25; CAS: 367-93-1
Phenylmethanesulfonyl fluoride (PMSF)	Sigma Aldrich	Cat#P7626; CAS: 329-98-6
Chelating Sepharose Fast Flow	GE Healthcare	Cat#17057502
Q Sepharose Fast Flow	GE Healthcare	Cat#17051001
Ubiquitin-AMC	VIVA Bioscience	Cat#VB2906-0050
Di-Ubiquitin K63-2	LifeSensors	Cat#DU6302
NSC-632839	LifeSensors	Cat#SI9689; CAS: 157654-67-6
SYPRO® Orange Protein Gel Stain	Thermo Fisher Scientific	Cat#S6650
vector pGEX-6p-1	GE Healthcare	Cat#28-9546-48
PreScission Protease	GE Healthcare	Cat# 27-0843-01
Recombinant USP2 (258-605)	This paper	N/A
Recombinant Ubiquitin (1-76)	This paper	N/A
Recombinant USP7 (208-561)	This paper	N/A
DUB enzymes and ubiquitin for MALDI TOF High Throughput DUB Activity Assay	(Ritorto et al., 2014)	N/A
Critical Commercial Assays
CytoTox-ONE™ Homogeneous Membrane Integrity Assay	Promega	Cat# G7891
Caspase-Glo® 3/7 Assay	Promega	Cat# G8091
GoTaq qPCR Master Mix	Promega	Cat# A6001
Experimental Models: Cell Lines
Human: HCT116	ECACC	Cat# 91091005, RRID:CVCL_0291
Human: U-2 OS	ECACC	Cat# 92022711, RRID:CVCL_0042
Human: SAOS-2	ECACC	Cat# 89050205, RRID:CVCL_0548
Human: MCF-7	ECACC	Cat# 86012803, RRID:CVCL_0031
Oligonucleotides
Human cyclin D1 siRNA	Santa Cruz Biotechnology	Cat# sc-29286
siRNA-A	Santa Cruz Biotechnology	Cat# sc-37007
siRNA-B	Santa Cruz Biotechnology	Cat# sc-44230
siRNA-C	Santa Cruz Biotechnology	Cat# sc-44231
Oligo-dT: TTTTTTTTTTTTTTT	Genomed	N/A
RT PCR CCND1 mRNA primer 1: TGCCAACCTCCTCAACGACCG	This paper	N/A
RT PCR CCND1 mRNA primer 2: TCGCAGACCTCCAGCATCCAG	This paper	N/A
RT PCR GAPDH mRNA primer 1: TGCACCACCAACTGCTTAGC	Genomed ; (Yin et al., 2001)	N/A
RT PCR GAPDH mRNA primer 2: GGCATGGACTGTGGTCATGAG	Genomed; (Yin et al., 2001)	N/A
Mycoplasma detection primer 1: GPO-1: ACTCCTACGGGAGGCAGCAGTA	Genomed; (van Kuppeveld et al., 1992)	N/A
Mycoplasma detection primer 2: MGSO: TGCACCATCTGTCACTCTGTTAACCTC	Genomed; (van Kuppeveld et al., 1992)	N/A
USP7 (208-561) primer forward: GCTACTCGAGTTACTATTCCTGCCGCTCC	Genomed	N/A
USP7 (208-561) primer reverse: GCAAGGATCCAAGAAGCACACAGGCTAC	Genomed	N/A
Recombinant DNA
pet16b-UB (1-76, human)	Prof. Stefan Jentsch, Max Planck Institute for Biochemistry, Munich, Germany; (Beers and Callis, 1993)	N/A
pet24a-USP2 (258-605, human)	Prof. Tad Holak, Max Planck Institute for Biochemistry, Munich, Germany; (Renatus et al., 2006)	N/A
human USP7 ORF	BioScience	OCABo5050A1122D
pGEX-6p-1-USP7 (208-561, human)	This paper	N/A
Software and Algorithms
ModFit LT Software v4.1.7	Verity Software House	http://www.vsh.com/products/mflt/index.asp
Image Lab v5.0	BioRad	http://www.bio-rad.com/en-us/product/image-lab-software
ImageJ 1.48v	Schneider et al., 2012	https://imagej.nih.gov/ij/