Fig 5. Loss of brain pericytes in adult Pdgfrβ^F7/F7 (F7/F7) mice.

(A, C, E) Representative confocal images of CD13-positive pericyte cell bodies co-localized with nuclear 4,6-Diamidino-2-phenylindole, dihydrochloride (DAPI) staining indicated by yellow arrows in the somatosensory cortex S1 region layers II-IV (S1Cx) (A), the CA1 subfield of the hippocampus (Hp) (C), and posterior thalamus (Thal) (E). Bar = 40 μm. (B, D, F) Quantification of CD13-positive cell bodies per mm² of tissue sections in S1Cx (B), Hp (D) and Thal (F). In each animal, 4–6 randomly selected fields in the cortex, hippocampus and thalamus were analyzed in 4 non-adjacent sections (~100 μm apart) and data were averaged per mouse. Mean ± S.E.M., n = 5 animals per group. In B, D, and F one-way ANOVA and Bonferroni’s post hoc tests were used to compare data in mutants versus age-matched littermate controls, and/or between different age groups of F7/F7 mutants. P < 0.05 indicates statistically significant differences between groups.