Fig 3. Anti-Ly6G-mediated depletion of neutrophils impairs control of pulmonary L. pneumophila infection.
B6 mice treated with isotype control (ISO) or anti-Ly6G (α-Ly6G) antibody were infected with ΔflaA L. pneumophila. A) Representative flow cytometry plots of lung cells from ISO or anti-Ly6G treated mice at 24 hours post-infection, with gates drawn around neutrophils and MCs. Total numbers of neutrophils (B) and Ly6Chi MCs (C) in the lung were quantified at 24 and 72 hours post-infection. (D) L. pneumophila CFUs in the lung were enumerated at 24 and 72 hours post-infection. Data shown are the pooled results of 4 independent experiments with 3 or 4 mice per group per experiment. * is p<0.05 and *** is p<0.001 by unpaired t-test. NS is not significant. Dashed line represents the limit of detection.