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. 2017 Apr 13;11(4):e0005513. doi: 10.1371/journal.pntd.0005513

Fig 2. RibJ is a novel family of riboflavin transporters characteristic of trypanosomatids.

Fig 2

(A) Multiple sequence alignment between Mch5p (S. cerevisiae), TcRibJ, TbRibJ and LmiRibJ using MUSCLE program. Red boxes: putative transmembrane domains; light blue box: MFS domain; green boxes: the Asn in N-glycosylation context. Black, gray and violet backgrounds represent 100%, 80%, and 60% conservation within similarity groups, respectively. (B) A Neighbor-joining tree (1000 pseudoreplicates) was constructed using the amino acid sequences from riboflavin transporters: 22 mammalian (RFVT/SLC52, light green), 15 fungal (Mch5p, yellow), 5 nematodal (rft, dark green) and 16 RibJ belonging to subclass Metakinetoplastids (light blue, and inside, trypanosomatids group in violet). The blue arrows indicate RibJ from T. cruzi, T. brucei and L. (L.) mexicana, and the human and S. cerevisiae riboflavin transporters.