Fig 4. TcRibJ functions as flavin transport in vivo in T. cruzi.

Epimastigotes transfected with pRIBOTEX-GFP (wt) or pRIBOTEX-TcRibJ (TcRibJ) were incubated in fresh SDM-20-10% FBS in the presence of (A) 20 nM riboflavin (RF), FMN or FAD, or (B) roseoflavin (RoF), lumiflavin (LF), or lumichrome (LC), at the indicated concentrations. Parasites were counted daily using a hemocytometer chamber. T. cruzi proliferation (%) was calculated at the third culture round, where the control condition (20 nM flavin) was referenced as 100%. (C) [³H]-riboflavin (100 nM) uptake measurements in control and over-expressing TcRibJ epimastigotes. Values are expressed as mean ± SD. (A-B) Statistical analysis was performed by a two-tailed unpaired t test (*P < 0.05, **P < 0.01, ***P < 0.005).