Fig 5. Cellular functions misregulated by HDACi after 48 h schistosomula treatment.

IPA analysis pointed to the “cell movement of smooth muscle cells” cellular function (A) that could be functionally limited due to the down-regulated transcription of all involved genes. (B) The cellular function “control of the quantity of reactive oxygen species (ROS)” may be decreased in TSA-treated parasites due to the fact that 20 out of 22 genes that encode proteins responsible for reducing free radical generation are down-regulated in schistosomula upon TSA treatment, and 2 out of 4 genes that increase ROS are up-regulated. Genes down-regulated by the HDACi treatment are colored in green and genes up-regulated are colored in red. Arrows indicate the predicted effect downstream of the gene according to the gene expression pattern; blue arrow shows a predicted inhibition of the function indicated at the center; orange arrow shows a predicted increase of the cellular function indicated at the center, and yellow arrows show inconsistent prediction of the function according to the gene expression pattern. S5 Table gives the corresponding Smp gene information for each gene symbol present in this figure.