Skip to main content
. 2017 Apr 13;11(4):e0005539. doi: 10.1371/journal.pntd.0005539

Fig 7. ChIP-qPCR targeting H3K9ac and total H3 at promoter region of differentially expressed genes.

Fig 7

Chromatin immunoprecipitation (ChIP) was performed with antibodies anti-H3K9ac (A) or anti-H3 (B) and DNA from schistosomula treated for 12 h with 1 μM TSA (solid bars) or with vehicle (open bars). DNA from the promoter regions of the indicated selected genes that was present in the immunoprecipitated fraction was quantified by qPCR with specific primers. Results are presented as % input DNA at the indicated target promoter region normalized by % input DNA at the promoter of the non-expressed reference gene SmVal19 (Smp_123090), as described in the Methods. Three independent biological replicates were analyzed. Statistical significance of enrichment was evaluated using t-test; asterisk indicates p-value ≤ 0.05.