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. 2017 Apr 13;11(4):e0005539. doi: 10.1371/journal.pntd.0005539

Fig 8. Synergistic effect of HDACi and EZH2i on schistosomula viability.

Fig 8

(A) Schistosomula were treated with drugs in five different conditions: control with vehicle (DMSO + Ethanol); 20 μM GSK343; 50 μM GSK343; 1 μM TSA and 1 μM TSA + 20 μM GSK343. Parasites were observed in the microscope for four days, and photographed with light and fluorescence microscopy. The number of dead parasites (stained in red with propidium iodide) was counted and compared with the total number of parasites (counted in the light microscope). The percentages of live viable schistosomula were calculated along the four days, with a different pool of parasites being stained and analyzed independently at each day. The experiment was performed with six biological replicates, and the mean +/- SD is shown; for each biological replicate, two technical replicates were obtained, and at least 100 schistosomula were counted for each technical replicate. Significant decrease in viability when comparing GSK343 alone and GSK343 plus TSA was assessed with a two-way ANOVA statistical test; asterisks indicate p-value ≤ 0.001. (B) Typical images at day 4 are shown. The calibration bar shows 100 μm.