Fig. 3.

Mice were treated with vehicle or 50 μg/kg/day for 28 days and the effect on bone architecture examined using μCT (a) and fluorescent bone labels (b). Further sections of bone were stained with H&E (c) or immunostained for cathepsin K (d), sclerostin (e) or periostin (f). Large images are 10 times and insert images are 40 times magnification. Scale bars in large images equals 100 μm and in insert images 50 μm. Osteoclasts were defined as large, strongly cathepsin K stained cells on a bone surface and are indicated by arrows in figure d. Examples of sclerostin positive osteocytes are indicated by arrows in figure e.