Table 1.

Construction of the plasmids for knockout of human ADAR1, MAVS and PKR using CRISPR/Cas9.

DOI: http://dx.doi.org/10.7554/eLife.25687.003

Genes	Primers		Nucleotide sequences (5’−3’)*	Targeting Region
ADAR1	sgADAR1-4	Forward	CACCGTCTGTCAAATGCCATATGGG	Exon2
		Reverse	AAACCCCATATGGCATTTGACAGAC
	sgADAR1-6	Forward	CACCGACTCAGTTCCTGGAAATGTG	Exon2
		Reverse	AAACCACATTTCCAGGAACTGAGTC
MAVS	sgMAVS-1	Forward	CACCGGAGGGCTGCCAGGTCAGAGG	Exon4
		Reverse	AAACCCTCTGACCTGGCAGCCCTC
PKR	sgPKR-1	Forward	CACCGTAATACATACCGTCAGAAGC	Exon1
		Reverse	AAACGCTTCTGACGGTATGTATTAC

*Nucleotides sequences which were used for targeting gene are indicated as bold and italic.