Table 2.
Primers for genotyping ADAR1, MAVS, and PKR knockout cells.
| Genes | Primers* | Nucleotide sequences (5’−3’) |
|---|---|---|
| ADAR1 | Forward-1 | ACCTTCCCTCCCAGGACTCCGGCC |
| Reverse-1 | CCTGAGTGGAGACCGCGATTTTCC | |
| Forward-2 | ATGGCCGAGATCAAGGAGAAAATC | |
| Reverse-2 | GTTCTGGTCTGGCCTCTTGCCTG | |
| MAVS | Forward | CTCCCCTGGCTCCTGTGCTCC |
| Reverse | AACTCCCTTTATTCCCACCTTG | |
| PKR | Forward | AGACTGAGATGAGTCCTATAAAG |
| Reverse | TCACCTATGAGTGAGAACATGC |
* Forward and Reverse-1 or -2 were used for sequencing of target region by sgADAR1-6 or sgADAR1-4 respectively.