Table 3.
Summary of native mass spectrometry.
| Protein sample | Concentration (μM) |
Oligomerization state | Measured mass ± error (Da)* | Calculated mass (Da) |
|---|---|---|---|---|
| yCAF1 | 2.5 | Cac1:Cac2:Cac3 | 174 492 ± 3 | 173965.1 |
| yCAF1 | 2.5 | Cac1:Cac3 | 123 927 ± 5 | 120735 |
| yCAF1 | 2.5 | Cac2:Cac3 | 103 840 ± 4 | 103755.1 |
| yCAF1 | 2.5 | Cac2 | 53 273 ± 6 | 53230.1 |
| yCAF1 | 2.5 | Cac3 | 50 568 ± 7 | 50525 |
| yCAF1-H3-H4 | 7 | Cac1:Cac2:Cac3:H3:H4 | 201 002 ± 5 | 200 720.3 |
| yCAF1-H3-H4 | 7 | Cac2:Cac3:H3:H4 | 130 343 ± 7 | 130510.4 |
| H3-H4 | 10† | (H3-H4)2 | 53 015 ± 4 | 53510.6 |
| H3-H4 | 10† | H3-H4 | 26 508 ± 2 | 26755.3 |
| H3-H4 | 10† | H3 | 15 271 ± 2 | 15388 |
| H3-H4 | 10† | H4 | 11236 ± 3 | 11367.3 |
*Values reported represent the mean value ± standard deviation according to (McKay et al., 2006). Combinations of neighboring m/z values were used to determine distinct M values of a macromolecule. Using these values, a mean value of M and its standard deviation were calculated.
†Values reported assume that H3-H4 are tetrameric in solution.